MeCP2 Antibody - N-terminal region (OAAB15948)

Catalog No: OAAB15948

Size:400UL

Western blot analysis of MeCP2 (arrow) using rabbit polyclonal MeCP2 Antibody (E11) (Cat. #OAAB15948). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MeCP2 gene.

MeCP2 Antibody (N-term) (Cat. # OAAB15948) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Primate
• Product Format: LiquidPBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: Flow cytometry, Western blot
• Additional Information: Cellular Location: Nucleus. Note=Colocalized with methyl-CpG in the genome
  Tissue Location: Present in all adult somatic tissues tested.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MeCP2.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Varies by lot. See vial for concentration.
• Intended Use: MeCP2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: MECP2
• Gene Full Name: methyl-CpG binding protein 2
• Alias Symbols: AUTSX3;meCp-2 protein;methyl-CpG-binding protein 2;MRX16;MRX79;MRXS13;MRXSL;PPMX;RS;RTS;RTT.
• NCBI Gene Id: 4204
• Protein Name: NP_001104262.1, NP_004983.1, P51608, Q95LG8
• Description of Target: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)- containing DNA, with a preference for 5-methylcytosine (5mC).
• Uniprot ID: https://www.uniprot.org/uniprot/?query=P51608+OR+Q95LG8
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001104262.1,NP_004983.1
• Molecular Weight: 52 kDa