MBNL1 ELISA Kit (Human) (OKEH08175)

Catalog No: OKEH08175

Size:96T

Human MBNL1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	107-118% (n = 20)	96-107% (n = 20)	109-117% (n = 20)	80-89% (n = 20)
  EDTA plasma(n=5)	105-114% (n = 20)	94-102% (n = 20)	93-105% (n = 20)	90-98% (n = 20)
  heparin plasma(n=5)	105-118% (n = 20)	86-95% (n = 20)	84-97% (n = 20)	85-97% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology MBNL1 ELISA Kit (Human) (OKEH08175) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MBNL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MBNL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MBNL1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.4% (n = 20)
  Mean Inter-assay CV%: <=7.0% (n = 20)
• ELISA Kit Component:
  • MBNL1 Microplate: 96 Wells (12 x 8 Well strips)
  • MBNL1 Lyophilized Standard: 2
  • 100X Biotinylated MBNL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
  Subcellular Location: Nucleus Cytoplasm Cytoplasmic granule Localized with DDX1, TIAL1 and YBX1 in stress granules upon stress (PubMed:18335541). Localized in the cytoplasm of multinucleated myotubes (PubMed:18335541). Colocalizes with nuclear foci of retained expanded-repeat transcripts in myotubes from patients affected by myotonic dystrophy (PubMed:10970838, PubMed:11590133, PubMed:11929853).
  Unigene: Hs.201858
  SMR: Q9NR56
  String: 9606.ENSP00000282486
  KEGG: hsa:4154
  MIM: 160900
  Pfam: PF00642
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.139ng/mL
• Specificity: Natural and recombinant Human Muscleblind-like protein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MBNL1
• Gene Full Name: muscleblind like splicing regulator 1
• Alias Symbols: EXP;MBNL;muscleblind-like;muscleblind-like protein 1;triplet-expansion RNA-binding protein.
• NCBI Gene Id: 4154
• Protein Name: Q9NR56
• Description of Target: Muscleblind-like protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q9NR56
• Protein Accession #: NP_001300986.1
• Nucleotide Accession #: NM_001314057.1