Lung Lysate (OCPB00065)

Catalog No: OCPB00065

Size:100UG

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Liquid. Lung lysate is supplied in SDS sample buffer containing 50 mM DTT.
• Host: Rat
• Application: Western blot
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 2 mg/ml
• Preparation: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.