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Catalog No: OKCD06285
Size:96WELLS
Price: $600.00
SKU
OKCD06285
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Datasheets/ManualsPrintable datasheet for LPL ELISA Kit (Human) (OKCD06285)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipoprotein lipase (LPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipoprotein lipase (LPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipoprotein lipase (LPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipoprotein lipase (LPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipoprotein lipase (LPL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipoprotein lipase (LPL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
LPL Microplate96 Wells (12 x 8 Well strips)
LPL Lyophilized Standard2
100X LPL HRP-Detector Anitbody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.226ng/mL
Publications

A novel NanoBiT-based assay monitors the interaction between lipoprotein lipase and GPIHBP1 in real time. J Lipid Res. 61, 546-559 (2020). 32029511

Description
Gene SymbolLPL
Gene Full Namelipoprotein lipase
Alias SymbolsHDLCQ11;LIPD;lipoprotein lipase;phospholipase A1.
NCBI Gene Id4023
Protein NameLipoprotein lipase
Description of TargetThe primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL) (PubMed:27578112). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity).
Uniprot IDP06858
Protein Accession #NP_000228.1
Nucleotide Accession #NM_000237.2
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