LIMA1 ELISA Kit (Human) (OKEH07986)

Catalog No: OKEH07986

Size:96T

Human LIMA1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology LIMA1 ELISA Kit (Human) (OKEH07986) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LIMA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LIMA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LIMA1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.6% (n = 20)
  Mean Inter-assay CV%: <=12.0% (n = 20)
• ELISA Kit Component:
  • LIMA1 Microplate: 96 Wells (12 x 8 Well strips)
  • LIMA1 Lyophilized Standard: 2
  • 100X Biotinylated LIMA1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
  Subcellular Location: Cytoplasm Cell junction Focal adhesion Cytoplasm Cytoskeleton This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.
  Unigene: Hs.525419
  SMR: Q9UHB6
  String: 9606.ENSP00000378400
  KEGG: hsa:51474
  MIM: 608364
  Pfam: PF00412
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.083ng/mL
• Specificity: Natural and recombinant Human LIM domain and actin-binding protein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LIMA1
• Gene Full Name: LIM domain and actin binding 1
• Alias Symbols: Epithelial protein lost in neoplasm;epithelial protein lost in neoplasm beta;EPLIN;LDLCQ8;LIM domain and actin-binding protein 1;SREBP3;sterol regulatory element binding protein 3.
• NCBI Gene Id: 51474
• Protein Name: Q9UHB6
• Description of Target: LIM domain and actin-binding protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UHB6
• Protein Accession #: NP_001107018.1
• Nucleotide Accession #: NM_001113546.1