LGSN ELISA Kit (Rabbit) (OKWB00407)

Catalog No: OKWB00407

Size:96T

Rabbit LGSN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-104%	91-103%	86-105%	86-105%
  EDTA Plasma (n=5)	82-100%	86-95%	84-99%	83-101%
  Heparin Plasma (n=5)	82-99%	80-100%	81-100%	80-98%

• ELISA Kit Principle: Aviva Systems Biology Lgsn ELISA Kit (Rabbit) (OKWB00407) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lgsn has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lgsn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lgsn captured in the well.
• ELISA Kit Range: 6.25-400ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	86-98	92
  EDTA Plasma (n=5)	89-101	94
  Heparin Plasma (n=5)	88-101	93

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Lgsn Microplate: 96 Wells (12 x 8 Well strips)
  • Lgsn Lyophilized Standard: 2 x 400 ng
  • 100X Lgsn Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 3.75 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: LGSN
• Gene Full Name: lengsin, lens protein with glutamine synthetase domain
• Alias Symbols: exostoses (multiple) 1;exostosin 1;GLULD1;glutamate-ammonia ligase domain-containing protein 1;lengsin;LGS.
• NCBI Gene Id: 100009041
• Protein Name: Lengsin
• Description of Target: May act as a component of the cytoskeleton or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens. Does not seem to have enzymatic activity (By similarity).
• Uniprot ID: Q0GA40
• Protein Accession #: NP_001075696
• Nucleotide Accession #: NM_001082227