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Catalog No: OKIA00224
Size:1 x 96 well plate
Price: $1,150.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHamster
Product Format12 of 8-well strips
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration170 minutes
ELISA Kit PrincipleThe Aviva Systems Biology Lgmn ELISA Kit (Hamster) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Hamster (CHO) Legumain in Tissue Culture Fluid. This assay employs an antibody specific for Hamster (CHO) Lgmn coated on a 96-well plate. Standards and samples are pipetted into the wells and Legumain present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Lgmn antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lgmn bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of Lgmn in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
ELISA Kit Range20 ng/mL - 0.3125 ng/ml
ELISA Kit Reproducibility< 10%
ELISA Kit Component
Anti-Hamster Lgmn Microplate96 Wells (12 x 8 Well Strips)
Hamster Lgmn Calibrator1
100X Lgmn Detection Antibody150 uL
100X Streptavidin-HRP Conjugate150 uL
5X Assay Diluent50 mL
20X Wash Solution50 mL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Additional InformationIntended Use: The Lgmn test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Lgmn/Legumain in Hamster (CHO) biological samples.
::Sample Volume: 5 uL
Reconstitution and StorageStore at 4 degrees
Sensitivity0.094 ng/mL
Alias SymbolsEC:;Asparaginyl Endopeptidase;Protease cysteine 1;
Protein NameLegumain
Description of TargetLegumain is a host cell protein (HCP) generated by CHO cells during production of biotherapeutics. This process-related impurity accumulates in the extracellular space as it is released from dead CHO cells and secreted from viable cells during cell culture. Legumain, a lysosomal protease, cleaves the asparaginyl and aspartyl bonds of therapeutic monoclonal antibodies, thus compromising their integrity, stability, and efficacy.
Uniprot IDG3I1H5
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