Lgmn ELISA Kit (Hamster) (OKIA00224)

Catalog No: OKIA00224

Size:1 x 96 well plate

Representative Hamster Lgmn Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Hamster
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 170 minutes
• ELISA Kit Principle: The Aviva Systems Biology Lgmn ELISA Kit (Hamster) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Hamster (CHO) Legumain in Tissue Culture Fluid. This assay employs an antibody specific for Hamster (CHO) Lgmn coated on a 96-well plate. Standards and samples are pipetted into the wells and Legumain present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Lgmn antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lgmn bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of Lgmn in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 20 ng/mL - 0.3125 ng/ml
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Hamster Lgmn Microplate: 96 Wells (12 x 8 Well Strips)
  • Hamster Lgmn Calibrator: 1
  • 100X Lgmn Detection Antibody: 150 uL
  • 100X Streptavidin-HRP Conjugate: 150 uL
  • 5X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The Lgmn test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Lgmn/Legumain in Hamster (CHO) biological samples.
  Sample Volume: 5 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 0.094 ng/mL

Target Info

• Alias Symbols: EC:3.4.22.34;Asparaginyl Endopeptidase;Protease cysteine 1;
• Protein Name: Legumain
• Description of Target: Legumain is a host cell protein (HCP) generated by CHO cells during production of biotherapeutics. This process-related impurity accumulates in the extracellular space as it is released from dead CHO cells and secreted from viable cells during cell culture. Legumain, a lysosomal protease, cleaves the asparaginyl and aspartyl bonds of therapeutic monoclonal antibodies, thus compromising their integrity, stability, and efficacy.
• Uniprot ID: G3I1H5