Leptin tA Protein (OPPA00618)

Catalog No: OPPA00618 (Formerly GWB-874A7C)

Size:10UG

Product Info

• Predicted Species Reactivity: Rat
• Product Format: The protein was lyophilized from a concentrated (0.85mg/ml) solution with 0.003mM NaHCO3. Physical appearance: White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Rat Recombinant in sterile water or sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted with other aqueous solutions.
  Biological Activity: Leptin Antagonist Triple Mutant Rat Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
  Protein Content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
  Protein Content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
  Product Description: Leptin Antagonist Triple Mutant Rat Recombinant is a singly non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Rat Recombinant was purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Leptin-Antagonist Triple Mutant Rat Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0. 1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP-tA mutant can be stored at 4C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0. 1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
• Purity: Greater than 99.0% as determined by (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Val - Pro - Ile - Gln.

Target Info

• Gene Symbol: rLeptin tA
• Alias Symbols: Leptin tA Rat, Leptin Antagonist Triple Mutant Rat Recombinant
• Description of Target: Recombinant Rat Leptin Triple Antagonist
• Protein Size (# AA): Recombinant