KLKB1 ELISA Kit (Human) (OKEH08687)

Catalog No: OKEH08687

Size:96T

Human KLKB1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	99-110% (n = 20)	105-114% (n = 20)	88-97% (n = 20)	88-100% (n = 20)
  EDTA plasma(n=5)	98-106% (n = 20)	98-108% (n = 20)	93-103% (n = 20)	105-114% (n = 20)
  heparin plasma(n=5)	101-111% (n = 20)	113-123% (n = 20)	93-105% (n = 20)	94-103% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology KLKB1 ELISA Kit (Human) (OKEH08687) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLKB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KLKB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLKB1 captured in well.
• ELISA Kit Range: 0.469-30ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.5% (n = 20)
  Mean Inter-assay CV%: <=7.7% (n = 20)
• ELISA Kit Component:
  • KLKB1 Microplate: 96 Wells (12 x 8 Well strips)
  • KLKB1 Lyophilized Standard: 2
  • 100X Biotinylated KLKB1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  Subcellular Location: Secreted
  Unigene: Hs.237642
  SMR: P03952
  String: 9606.ENSP00000264690
  KEGG: hsa:3818
  MIM: 229000
  Pfam: PF00024
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.235ng/mL
• Specificity: Natural and recombinant Human Plasma kallikrein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KLKB1
• Gene Full Name: kallikrein B1
• Alias Symbols: Fletcher factor;kallikrein B, plasma (Fletcher factor) 1;kininogenin;KLK3;PKK;PKKD;plasma kallikrein;plasma prekallikrein;PPK.
• NCBI Gene Id: 3818
• Protein Name: P03952
• Description of Target: Plasma kallikrein
• Uniprot ID: https://www.uniprot.org/uniprot/P03952
• Protein Accession #: NP_000883.2
• Nucleotide Accession #: NM_000892.4