KIF5A ELISA Kit (Human) (OKCD00976)

Catalog No: OKCD00976 (Formerly GWB-KBBEQ8)

Size:96WELLS

Human KIF5A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 5A (KIF5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin Family, Member 5A (KIF5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 5A (KIF5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kinesin Family, Member 5A (KIF5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kinesin Family, Member 5A (KIF5A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kinesin Family, Member 5A (KIF5A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KIF5A Microplate: 96 Wells (12 x 8 Well Strips)
  • KIF5A Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated KIF5A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.112 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kinesin Family, Member 5A (KIF5A). No significant cross-reactivity or interference between Kinesin Family, Member 5A (KIF5A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KIF5A
• Gene Full Name: kinesin family member 5A
• Alias Symbols: ALS25;D12S1889;KIF5A variant protein;kinesin heavy chain isoform 5A;kinesin heavy chain neuron-specific 1;kinesin, heavy chain, neuron-specific;MY050;NEIMY;neuronal kinesin heavy chain;NKHC;SPG10.
• NCBI Gene Id: 3798
• Protein Name: Kinesin heavy chain isoform 5A
• Description of Target: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons.
• Uniprot ID: Q12840
• Protein Accession #: NP_004975
• Nucleotide Accession #: NM_004984.2
• Protein Size (# AA): 1032