JUP ELISA Kit (Bovine) (OKEH08147)

Catalog No: OKEH08147

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology JUP ELISA Kit (Bovine) (OKEH08147) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for JUP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for JUP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample JUP captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.3% (n = 20)
  Mean Inter-assay CV%: <=7.0% (n = 20)
• ELISA Kit Component:
  • JUP Microplate: 96 Wells (12 x 8 Well strips)
  • JUP Lyophilized Standard: 2
  • 100X Biotinylated JUP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  Subcellular Location: Cell junction Adherens junction Cell junction Desmosome Cytoplasm Cytoskeleton Membrane Peripheral membrane protein Cytoplasmic in a soluble and membrane-associated form.
  Unigene: Bt.20230
  SMR: Q8SPJ1
  String: 9913.ENSBTAP00000023522
  KEGG: bta:445543
  Pfam: PF00514
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Junction plakoglobin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: JUP
• Gene Full Name: junction plakoglobin
• Alias Symbols: desmoplakin III;desmoplakin-3;junction plakoglobin.
• NCBI Gene Id: 445543
• Protein Name: Q8SPJ1
• Description of Target: Junction plakoglobin
• Uniprot ID: https://www.uniprot.org/uniprot/Q8SPJ1
• Protein Accession #: NP_001004024.1
• Nucleotide Accession #: NM_001004024.3