IRGM Antibody (OAPB00657)

Catalog No: OAPB00657

Size:100UG

Western blot analysis of IRGM in human brain lysate with IRGM antibody at (A) 1 and (B) 2 ug/mL.

Western blot analysis of IRGM in human brain lysate with IRGM antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry of IRGM in human brain with IRGM antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. IRGM Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Brain Tissue Lysate
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: IRGM antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: IRGM antibody was raised against a 14 amino acid synthetic peptide near the center of the human IRGM. The immunogen is located within the last 50 amino acids of IRGM.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-IRGM antibody is Catalog # OEPB02777
• Application Info: IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.;Massey DC and Parkes M. Genome-wide association scanning highlights two autophagy genes, ATG16L1 and IRGM, as being significantly associated with Crohn's disease. Autophagy2007; 3:649-51.;Fisher SA, Tremelling M, Anderson CA, et al. Genetic determinants of ulcerative colitis include the ECM1 locus and five loci implicated in Crohn's disease. Nat. Genet.2008; 40:710-2. ;Singh SB, Davis AS, Taylor GA, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science2006; 313:1438-41.

Target Info

• Gene Symbol: IRGM
• Gene Full Name: immunity related GTPase M
• Alias Symbols: IFI1;immunity-related GTPase family M protein;Immunity-related GTPase family M protein 1;immunity-related GTPase family, M1;interferon-inducible protein 1;IRGM1;LPS-stimulated RAW 264.7 macrophage protein 47 homolog;LRG47;LRG-47;LRG-47-like protein.
• NCBI Gene Id: 345611
• Protein Name: Immunity-related GTPase family M protein|IRGM protein [Homo sapiens]
• Description of Target: Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility (By similarity).
• Uniprot ID: A1A4Y4
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAI28169
• Molecular Weight: Predicted: 20 kDa Observed: 24 kDa