ILF2 ELISA Kit (Human) (OKEH08335)

Catalog No: OKEH08335

Size:96T

Human ILF2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology ILF2 ELISA Kit (Human) (OKEH08335) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ILF2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ILF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ILF2 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.0% (n = 20)
  Mean Inter-assay CV%: <=6.4% (n = 20)
• ELISA Kit Component:
  • ILF2 Microplate: 96 Wells (12 x 8 Well strips)
  • ILF2 Lyophilized Standard: 2
  • 100X Biotinylated ILF2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. Essential for the efficient reshuttling of ILF3 (isoform 1 and isoform 2) into the nucleus.
  Subcellular Location: Nucleus Nucleolus Cytoplasm Nucleus Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  Unigene: Hs.75117
  SMR: Q12905
  String: 9606.ENSP00000355011
  KEGG: hsa:3608
  MIM: 603181
  Pfam: PF07528
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.085ng/mL
• Specificity: Natural and recombinant Human Interleukin enhancer-binding factor 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ILF2
• Gene Full Name: interleukin enhancer binding factor 2
• Alias Symbols: interleukin enhancer binding factor 2, 45kD;interleukin enhancer binding factor 2, 45kDa;interleukin enhancer-binding factor 2;NF45;Nuclear factor of activated T-cells 45 kDa;nuclear factor of activated T-cells, 45-kDa;PRO3063.
• NCBI Gene Id: 3608
• Protein Name: Q12905
• Description of Target: Interleukin enhancer-binding factor 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q12905
• Protein Accession #: NP_001254738.1
• Nucleotide Accession #: NM_001267809.1