IL5RA ELISA Kit (Mouse) (OKCD07455)

Catalog No: OKCD07455

Size:96WELLS

IL5RA ELISA Kit (Mouse) (OKCD07455) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 Receptor Alpha (IL5Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 5 Receptor Alpha (IL5Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 5 Receptor Alpha (IL5Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 5 Receptor Alpha (IL5Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 62.50-4000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 5 Receptor Alpha (IL5Ra) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 5 Receptor Alpha (IL5Ra) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IL5RA Microplate: 96 Wells (12 x 8 Well strips)
  • IL5RA Lyophilized Standard: 2
  • 100X IL5RA HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 28.5pg/mL

Target Info

• Gene Symbol: Il5ra
• Gene Full Name: interleukin 5 receptor, alpha
• Alias Symbols: CD125;CDw125;I;IL-5 receptor alpha chain;IL-5 receptor subunit alpha;Il5r;IL-5R subunit alpha;interleukin-5 receptor subunit alpha.
• NCBI Gene Id: 16192
• Protein Name: Interleukin-5 receptor subunit alpha
• Description of Target: This is the receptor for interleukin-5. The alpha chain binds to IL5.
• Uniprot ID: P21183
• Protein Accession #: NP_032396.1
• Nucleotide Accession #: NM_008370.2