IgA ELISA Kit (Human) (OKGD00009)

Catalog No: OKGD00009

Size:96 Wells

Human IgA ELISA Kit standard curve.

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~2.5 hours
• ELISA Kit Principle: Aviva Systems Biology IGA ELISA Kit (Human) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGA has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for IGAis added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGA captured in well.
• ELISA Kit Range: 1.95 - 250 ng/mL
• ELISA Kit Component:
  • Anti-IgA Microplate: 1 x 96 wells plate
  • IgA Lyophilized Standard: 2 x vials (250 ng/vial)
  • IgA HRP-Detection Antibody: 1 x 10 uL
  • Assay Diluent A: 1 x 30 mL
  • 20X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 12 mL
  • Stop Solution: 1 x 12 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, and other biological fluids
• Sensitivity: <= 1.0 ng/mL
• Specificity:

  Species	Cross Reactivity (%)
  Chicken	0
  Dog	0
  Mouse	0
  Pig	0
  Rat	0

  Antigen	Cross Reactivity (%)
  AAT	0
  APOA1	0
  A2M	0
  C3	0
  Fibrinogen	0
  HP	0
  HDL	0
  H. HSA	0
  IgG	0
  IgM	0
  LDL	0
  Orosomucoid	0
  Transferrin	0

Target Info

• Gene Symbol: IGA
• Gene Full Name: Immunoglobulin heavy constant alpha 1 and 2
• Alias Symbols: IgA1
• NCBI Gene Id: 3493
• Protein Name: Immunoglobulin heavy constant alpha 1
• Description of Target: Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).
• Uniprot ID: P01876