HSPBAP1 ELISA Kit (Human) (OKWB00229)

Catalog No: OKWB00229

Size:96T

Human HSPBAP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	89-101%	85-105%	86-95%	86-99%
  EDTA Plasma (n=5)	84-96%	87-101%	87-99%	82-96%
  Heparin Plasma (n=5)	83-99%	80-99%	82-100%	86-92%

• ELISA Kit Principle: Aviva Systems Biology HSPBAP1 ELISA Kit (Human) (OKWB00229) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPBAP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPBAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPBAP1 captured in the well.
• ELISA Kit Range: 0.313-20ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	89-99	94
  EDTA Plasma (n=5)	86-103	93
  Heparin Plasma (n=5)	90-101	95

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • HSPBAP1 Microplate: 96 Wells (12 x 8 Well strips)
  • HSPBAP1 Lyophilized Standard: 2 x 20 ng
  • 100X HSPBAP1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.188 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HSPBAP1
• Gene Full Name: HSPB1 associated protein 1
• Alias Symbols: 27 kDa heat shock protein-associated protein 1;HSPB (heat shock 27kDa) associated protein 1;HSPB1-associated protein 1;PASS1;Protein associated with small stress protein 1;protein associating with small stress protein PASS1.
• NCBI Gene Id: 79663
• Protein Name: HSPB1-associated protein 1
• Description of Target: May play a role in cellular stress response.
• Uniprot ID: Q96EW2
• Protein Accession #: NP_001307657
• Nucleotide Accession #: NM_001320728