HSD17B4 Antibody (OAGA01102)

Catalog No: OAGA01102

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
OAGA01102 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
OAGA01102 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded OVCA, using HSD17B4(OAGA01102) antibody at 1:500 dilution.

HSD17B4 antibody detects HSD17B4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: HSD17B4 protein stained by HSD17B4 antibody (OAGA01102) diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Positive Controls: HeLa, mouse brain
  Cellular Localization: Peroxisome
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 18 and 261 of HSD17B4 (Uniprot ID#P51659)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.66 mg/ml
• Application Info: Suggested starting dilutions are as follows:
  WB: 1:500-1:3000,
  IHC-FFPE: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)],
  ICC/IF: 1:100-1:1000.
  Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: HSD17B4
• Gene Full Name: Peroxisomal multifunctional enzyme type 2
• Alias Symbols: DBP, MFE-2, MFP-2, MPF-2, PRLTS1, SDR8C1
• NCBI Gene Id: 3295
• Protein Name: Peroxisomal multifunctional enzyme type 2
• Description of Target: The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq]
• Uniprot ID: P51659
• Protein Accession #: NP_000405.1
• Molecular Weight: 80 kDa