HSD11B2 ELISA Kit (Rat) (OKCD01261)

Catalog No: OKCD01261 (Formerly GWB-KBBDE7)

Size:96WELLS

Rat Hsd11b2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	91-101%	82-93%	80-103%	79-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	79-102	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Hsd11b2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Hsd11b2 Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated Hsd11b2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.30 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2). No significant cross-reactivity or interference between 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Hsd11b2
• Gene Full Name: hydroxysteroid 11-beta dehydrogenase 2
• Alias Symbols: 11-beta-HSD2;11-beta-hydroxysteroid dehydrogenase type 2;11-DH2;corticosteroid 11-beta-dehydrogenase isozyme 2;Hydroxysteroid dehydrogenase, 11 beta type 2;NAD-dependent 11-beta-hydroxysteroid dehydrogenase.
• NCBI Gene Id: 25117
• Protein Name: Corticosteroid 11-beta-dehydrogenase isozyme 2
• Description of Target: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.
• Uniprot ID: P50233
• Protein Accession #: NP_058777
• Nucleotide Accession #: NM_017081.2
• Protein Size (# AA): 400