HNRNPA2B1 ELISA Kit (Human) (OKCD06245)

Catalog No: OKCD06245

Size:96WELLS

HNRNPA2B1 ELISA Kit (Human) (OKCD06245) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRPA2B1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HNRNPA2B1 Microplate: 96 Wells (12 x 8 Well strips)
  • HNRNPA2B1 Lyophilized Standard: 2
  • 100X HNRNPA2B1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.058ng/mL

Target Info

• Gene Symbol: HNRNPA2B1
• Gene Full Name: heterogeneous nuclear ribonucleoprotein A2/B1
• Alias Symbols: epididymis secretory sperm binding protein;heterogeneous nuclear ribonucleoproteins A2/B1;hnRNP A2 / hnRNP B1;HNRNPA2;HNRNPA2B1/MYC fusion;HNRNPB1;HNRPA2;HNRPA2B1;HNRPB1;IBMPFD2;nuclear ribonucleoprotein particle A2 protein;RNPA2;SNRPB1.
• NCBI Gene Id: 3181
• Protein Name: Heterogeneous nuclear ribonucleoproteins A2/B1
• Description of Target: Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. Packaging plays a role in various processes such as transcription, pre-mRNA processing, RNA nuclear export, subcellular location, mRNA translation and stability of mature mRNAs (PubMed:19099192). Forms hnRNP particles with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm (PubMed:10567417). Specifically binds single-stranded telomeric DNA sequences, protecting telomeric DNA repeat against endonuclease digestion (By similarity). Also binds other RNA molecules, such as primary miRNA (pri-miRNAs): acts as a nuclear 'reader' of the N6-methyladenosine (m6A) mark by specifically recognizing and binding a subset of nuclear m6A-containing pri-miRNAs. Binding to m6A-containing pri-miRNAs promotes pri-miRNA processing by enhancing binding of DGCR8 to pri-miRNA transcripts (PubMed:26321680). Involved in miRNA sorting into exosomes following sumoylation, possibly by binding (m6A)-containing pre-miRNAs (PubMed:24356509). Acts as a regulator of efficiency of mRNA splicing, possibly by binding to m6A-containing pre-mRNAs (PubMed:26321680).
• Uniprot ID: P22626
• Protein Accession #: NP_002128.1
• Nucleotide Accession #: NM_002137.3