- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
HNF4A Antibody - middle region (OASG03561)
| Datasheets/Manuals | Printable datasheet for anti-HNF4A (OASG03561) antibody |
|---|
| Predicted Species Reactivity | Human, Mouse, Rat |
|---|---|
| Product Format | Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | WB, IHC-P, ELISA |
| Additional Information | Cellular Localization: Nucleus |
| Reconstitution and Storage | Store at -20°C. Avoid repeated freeze/thaw cycles. |
| Immunogen | Synthesized peptide derived from the Internal region of human HNF-4 Alpha/Gamma. Location: 60-140aa. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Target Post-Translational Modification | Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. Acetylation at Lys-458 lowers transcriptional activation by about two-fold. |
| Specificity | HNF-4 Alpha/Gamma Polyclonal Antibody detects endogenous levels of HNF-4 Alpha/Gamma protein. |
| Application Info | WB: 1:500~2000 IHC: 1:100~300 ELISA: 1:20000 |
| Gene Symbol | HNF4A, HNF4G |
|---|---|
| Gene Full Name | hepatocyte nuclear factor 4, alpha |
| Alias Symbols | TCF, HNF4, MODY, FRTS4, MODY1, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, HNF4alpha |
| NCBI Gene Id | 3172; 3174; 15378; 30942; 25735 |
| Description of Target | Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells. |
| Uniprot ID | P41235. |
| Molecular Weight | 52 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "HNF4A Antibody - middle region (OASG03561)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
-
How long will it take to receive "HNF4A Antibody - middle region (OASG03561)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
-
What buffer format is "HNF4A Antibody - middle region (OASG03561)" provided in?
This item is provided in "Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HNF4A Antibody - middle region (OASG03561)"?
This target may also be called "TCF, HNF4, MODY, FRTS4, MODY1, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, HNF4alpha" in publications.
-
What is the shipping cost for "HNF4A Antibody - middle region (OASG03561)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "HNF4A Antibody - middle region (OASG03561)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "HNF4A Antibody - middle region (OASG03561)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "52 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HNF4A Antibody - middle region (OASG03561)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "HNF4A, HNF4G"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "HNF4A, HNF4G"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "HNF4A, HNF4G"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "HNF4A, HNF4G"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "HNF4A, HNF4G"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "HNF4A, HNF4G"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.