HMMR ELISA Kit (Human) (OKCD07971)

Catalog No: OKCD07971

Size:96WELLS

HMMR ELISA Kit (Human) (OKCD07971) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronan Mediated Motility Receptor (HMMR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronan Mediated Motility Receptor (HMMR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronan Mediated Motility Receptor (HMMR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Hyaluronan Mediated Motility Receptor (HMMR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronan Mediated Motility Receptor (HMMR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronan Mediated Motility Receptor (HMMR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HMMR Microplate: 96 Wells (12 x 8 Well strips)
  • HMMR Lyophilized Standard: 2
  • 100X HMMR HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.057ng/mL

Target Info

• Gene Symbol: HMMR
• Gene Full Name: hyaluronan mediated motility receptor
• Alias Symbols: CD168;hyaluronan mediated motility receptor;IHABP;intracellular hyaluronic acid-binding protein;receptor for hyaluronan-mediated motility;RHAMM.
• NCBI Gene Id: 3161
• Protein Name: Hyaluronan mediated motility receptor
• Description of Target: Receptor for hyaluronic acid (HA) (By similarity). Involved in cell motility (By similarity). When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis (By similarity).
• Uniprot ID: O75330
• Protein Accession #: NP_001136028.1
• Nucleotide Accession #: NM_001142556.1