HLA-G ELISA Kit (Human) (OKCD07736)

Catalog No: OKCD07736

Size:96WELLS

HLA-G ELISA Kit (Human) (OKCD07736) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I G (MHCG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I G (MHCG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I G (MHCG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Major Histocompatibility Complex Class I G (MHCG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.38-24ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Major Histocompatibility Complex Class I G (MHCG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Major Histocompatibility Complex Class I G (MHCG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HLA-G Microplate: 96 Wells (12 x 8 Well strips)
  • HLA-G Lyophilized Standard: 2
  • 100X HLA-G HRP-Detector Anitbody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.17ng/mL

Target Info

• Gene Symbol: HLA-G
• Gene Full Name: major histocompatibility complex, class I, G
• Alias Symbols: b2 microglobulin;HLA class I histocompatibility antigen, alpha chain G;HLA G antigen;HLA-G histocompatibility antigen, class I, G;MHC class I antigen G;MHC class Ib antigen;MHC-G;mutant MHC class I antigen;mutant MHC class Ib antigen.
• NCBI Gene Id: 3135
• Protein Name: HLA class I histocompatibility antigen, alpha chain G
• Description of Target: Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.
• Uniprot ID: P17693
• Protein Accession #: NP_002118.1
• Nucleotide Accession #: NM_002127.5