HIST2H2AA4 Antibody (Acetyl-Lys5) (OAGA07364)

Catalog No: OAGA07364

Size:100 ul

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein by Western blot analysis.
A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H2AK5ac (acetyl Lys5) antibody dilution: 1:10000

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 0.4 uM TSA for 18 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Histone H2AK5ac (acetyl Lys5) antibody immunoprecipitates Histone H2AK5ac (acetyl Lys5) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Histone H2AK5ac (acetyl Lys5) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2AK5ac (acetyl Lys5) protein by 2.5 ug of Histone H2AK5ac (acetyl Lys5) antibody 12% SDS-PAGE The immunoprecipitated Histone H2AK5ac (acetyl Lys5) protein was detected by Histone H2AK5ac (acetyl Lys5) antibody diluted at 1 : 10000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Dotblot analysis of anti-Histone H2AK5ac (acetyl Lys5) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2AK5ac (acetyl Lys5) antibody 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: DB, ICC, IF, IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Target Post-Translational Modification: Acetyl-Lys5
• Application Info: Suggested starting dilutions are as follows: Dot, ICC/IF: 1:100-1:1000, IP: 1:100-1:10000, WB: 1:1000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: HIST2H2AA4
• Gene Full Name: histone cluster 2 H2A family member a4
• Alias Symbols: H2A/R, H2AC18, HIST2H2AA4
• NCBI Gene Id: 723790; 8337;
• Protein Name: histone H2A type 2-A
• Description of Target: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.
• Uniprot ID: Q6FI13
• Protein Accession #: NP_001035807.1
• Nucleotide Accession #: NM_001040874.1