HIST1H4B ELISA Kit (Rat) (OKCD09442)

Catalog No: OKCD09442

Size:96WELLS

HIST1H4B ELISA Kit (Rat) (OKCD09442) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Cluster 1, H4a (HIST1H4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Cluster 1, H4a (HIST1H4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Cluster 1, H4a (HIST1H4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone Cluster 1, H4a (HIST1H4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Cluster 1, H4a (HIST1H4A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Cluster 1, H4a (HIST1H4A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HIST1H4B Microplate: 96 Wells (12 x 8 Well strips)
  • HIST1H4B Lyophilized Standard: 2
  • 100X HIST1H4B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.069ng/mL

Target Info

• Gene Symbol: H4c3|Hist1h2ao|Hist1h4b|Hist1h4m|Hist2h4|Hist2h4a|LOC100912564|RGD1562378
• Gene Full Name: H4 clustered histone 3|histone cluster 1 H4 family member B|histone cluster 1, H2ao|histone cluster 1, H4m|histone cluster 2 H4 family member A|histone cluster 2, H4|histone H4 variant H4-v.1|histone H4-like
• Alias Symbols: germinal histone H4;H1ft;H4c2;H4f16;Hist1h4b;Hist4;Hist4h4;histone 1, H2ao;histone 1, H4m;histone 2, H4;histone cluster 1, H4b;histone cluster 2, H4;histone H2A type 1-E;histone H4;histone H4-like;RGD1565073.
• NCBI Gene Id: 100912489|100912564|291152|295277|364723|500351|502913|64627
• Protein Name: Histone H4
• Description of Target: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• Uniprot ID: P62804
• Protein Accession #: NP_001116941.1
• Nucleotide Accession #: NM_001123469.1