His-Tag Polyclonal Antibody (OAEC00713)

Catalog No: OAEC00713

Size:50ug

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Western blot analysis of extract from control 293 cells (Lane 1) or transfected with His-tagged S6 (Lane 2) and transfected with His-tagged CREB (Lane 3) using His-Tag polyclonal antibody (OAEC00713).

Product Info

• Predicted Species Reactivity: All
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: Store at -20C/1 year
• Immunogen: The antiserum was produced by immunizing rabbit with synthesized peptide containing the His epitope (H-H-H-H-H-H).
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
• Concentration: 1 mg/ml
• Specificity: His-Tag polyclonal antibody detects His-tagged proteins overexpressed in cells. The antibody recognizes the His-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:500-1:1000
• Reference: Terpe K. et al. (2003) Appl Microbiol Biotechnol; 60(5):523-533.Xu L. et al. (2006) Cell Mol Immunol; 3(2):139-143.Els Conrath, K., et al. ( 2001) J. Biol. Chem. 276: 7346-7350.Western blot analysis of extract from control 293 cells (Lane 1) or transfected with His-tagged S6 (Lane 2) and transfected with His-tagged CREB (Lane 3) using His-Tag polyclonal antibody (E0T502).For Research