HIRA Antibody (Phospho-Thr555) (OASG03337)

Catalog No: OASG03337

Size:100 uL

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC, IF
  Function: Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
  Cellular Localization: Nucleus. Nucleus, PML body. Note=Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
  Tissue Specificity: Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human HIRA around the phosphorylation site of T555. Location: 490-570aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Sumoylated. Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
• Specificity: Phospho-HIRA (T555) Polyclonal Antibody detects endogenous levels of HIRA protein only when phosphorylated at T555.
• Application Info: IHC: 1:100~300
  IF: 1:200~1000
  ELISA: 1:10000

Target Info

• Gene Symbol: HIRA
• Gene Full Name: histone cell cycle regulator
• Alias Symbols: TUP1, DGCR1, TUPLE1
• NCBI Gene Id: 7290; 15260;
• Description of Target: This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
• Uniprot ID: P54198, Q61666