HIF1A Antibody (OASG03329)

Catalog No: OASG03329

Size:100 uL

Western Blot

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB
• Additional Information: Cellular Localization: Cytoplasm Nucleus Nucleus speckle. Colocalizes with HIF3A in the nucleus and speckles. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.
  Tissue Specificity: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Recombinant Protein
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
• Concentration: 1 mg/ml
• Specificity: The antibody detects endogenous HIF-1 Alpha protein.
• Application Info: WB: 1:1000~2000

Target Info

• Gene Symbol: HIF1A
• Gene Full Name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Alias Symbols: HIF1, MOP1, PASD8, HIF-1A, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF-1-alpha
• NCBI Gene Id: 3091; 15251; 29560
• Description of Target: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
• Uniprot ID: Q16665, Q61221, O35800