Catalog No: OKCA00465
Size:96 Wells
Price: $754.00
SKU
OKCA00465
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Heat Shock Protein 70 Antibody (IgG) (Human) ELISA Kit (OKCA00465)
Predicted Species Reactivity | Human | ||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Heat Shock Protein 70 Antibody (IgG) (Human) ELISA Kit (OKCA00465) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Heat Shock Protein 70 antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanIgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Heat Shock Protein 70 Antibody (IgG) captured in well. | ||||||||||||||||||||
ELISA Kit Reproducibility | Three different samples of known Heat Shock Protein 70 Antibody (IgG) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each. Intra-Assay CV: <15% Inter-Assay CV: <15% | ||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||
Sample Type | Serum and Plasma | ||||||||||||||||||||
Assay Info | Assay Methodology: Qualitative Reverse Capture ELISA | ||||||||||||||||||||
Protocol Information |
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Alias Symbols | HSP-70 |
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Protein Name | Heat Shock Protein 70 Antibody (IgG) |
Description of Target | Heat Shock Protein 70 Antibody (IgG) |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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