Haptoglobin ELISA Kit (Sheep) (OKIA00188)

Catalog No: OKIA00188

Size:1x96WELLPLATE

Product Info

• Predicted Species Reactivity: Sheep
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 55 Minutes
• ELISA Kit Principle: Aviva Systems Biology Haptoglobin ELISA Kit (Sheep) (OKIA00188) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Haptoglobin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Haptoglobin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Haptoglobin captured in the well.
• ELISA Kit Range: 15.625 ng/ml - 1000 ng/ml
• ELISA Kit Component:
  • Anti-Sheep Haptoglobin Microplate: 96 Wells (12 x 8 Well Strips)
  • 20X Diluent Concentrate: 50 mL
  • 20X Wash Solution: 50 mL
  • 100X Enzyme-Antibody Conjugate: 150 uL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
  • Sheep Haptoglobin Calibrator: 1
• Additional Information: Intended Use: The Hp test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Hp in Sheep biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 5.047 ng/ml
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Alias Symbols: HP
• Protein Name: Haptoglobin