H3-4 ELISA Kit (Human) (OKEH08623)

Catalog No: OKEH08623

Size:96T

Human H3-4 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	103-116% (n = 20)	97-106% (n = 20)	101-111% (n = 20)	92-102% (n = 20)
  EDTA plasma(n=5)	93-104% (n = 20)	87-99% (n = 20)	102-112% (n = 20)	92-104% (n = 20)
  heparin plasma(n=5)	88-96% (n = 20)	112-120% (n = 20)	99-107% (n = 20)	96-106% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology H3-4 ELISA Kit (Human) (OKEH08623) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H3-4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H3-4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H3-4 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=9.1% (n = 20)
  Mean Inter-assay CV%: <=10.6% (n = 20)
• ELISA Kit Component:
  • H3-4 Microplate: 96 Wells (12 x 8 Well strips)
  • H3-4 Lyophilized Standard: 2
  • 100X Biotinylated H3-4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  Subcellular Location: Nucleus Chromosome
  Unigene: Hs.248171
  SMR: Q16695
  String: 9606.ENSP00000355657
  KEGG: hsa:8290
  MIM: 602820
  Pfam: PF00125
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.042ng/mL
• Specificity: Natural and recombinant Human Histone H3.1t
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: H3-4
• Gene Full Name: H3.4 histone
• Alias Symbols: H3 histone family, member T;H3.4;H3/g;H3/t;H3FT;H3t;HIST3H3;histone 3, H3;histone cluster 3 H3;histone H3.1t;histone H3.4.
• NCBI Gene Id: 8290
• Protein Name: Q16695
• Description of Target: Histone H3.1t
• Uniprot ID: https://www.uniprot.org/uniprot/Q16695
• Protein Accession #: NP_003484.1
• Nucleotide Accession #: NM_003493.2