GSTA1 ELISA Kit (Mouse) (OKCD00806)

Catalog No: OKCD00806 (Formerly GWB-KBBOQ6)

Size:96WELLS

Mouse Gsta1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	83-90%	82-105%	86-94%	87-99%
  EDTA plasma(n=5)	80-93%	81-95%	80-102%	81-98%
  heparin plasma(n=5)	87-104%	97-104%	79-105%	94-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Alpha 1 (GSTa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Alpha 1 (GSTa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Alpha 1 (GSTa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione S Transferase Alpha 1 (GSTa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	102
  EDTA plasma(n=5)	79-99	93
  heparin plasma(n=5)	87-101	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Alpha 1 (GSTa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Alpha 1 (GSTa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Gsta1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Gsta1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Gsta1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.129 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Alpha 1 (GSTa1). No significant cross-reactivity or interference between Glutathione S Transferase Alpha 1 (GSTa1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Gsta1
• Gene Full Name: glutathione S-transferase, alpha 1 (Ya)
• Alias Symbols: 13-hydroperoxyoctadecadienoate peroxidase;androst-5-ene-3,17-dione isomerase;glutathione S-transferase A1;glutathione S-transferase Ya;glutathione S-transferase Ya1;GST class-alpha member 1;Gst2-;Gst2-1.
• NCBI Gene Id: 14857
• Protein Name: Glutathione S-transferase A1
• Description of Target: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
• Uniprot ID: P13745
• Protein Accession #: NP_032207
• Nucleotide Accession #: NM_008181.3
• Protein Size (# AA): 223