GRP ELISA Kit (Mouse) (OKCA02384)

Catalog No: OKCA02384

Size:96 Wells

Mouse GRP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	98	92-102
  1:2	98	91-106
  1:4	95	89-98
  1:8	96	92-104

• ELISA Kit Principle: Aviva Systems Biology Grp ELISA Kit (Mouse) (OKCA02384) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grp has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Grp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Grp captured in the well.
• ELISA Kit Range: 39.063 - 2,500 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	94	89-98
  EDTA Plasma (n=4)	89	82-96

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Grp Microplate: 96 Wells (12 x 8 Well strips)
  • Grp Lyophilized Standard: 2
  • 100X Biotinylated Grp Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 9.77 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: GRP
• Alias Symbols: B, BLP
• NCBI Gene Id: 225642
• Protein Name: Gastrin-releasing peptide
• Description of Target: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP-receptor gene expression, long-term potentiation, and amygdala-dependent memory for fear.
• Uniprot ID: Q8R1I2
• Protein Accession #: NP_778177
• Nucleotide Accession #: NM_175012