GPR119 Antibody (OAAI00430)

Catalog No: OAAI00430

Size:100UG

Western Blot: The Cell lysate derived from MS1 ( Lane 1) and Mouse brain (Lane 2) were resolved onto 1% SDS- PAGE, transferred onto NC membrane, and immuno- blotted by Rb anti GPR119 (Cat#OAAI00430) at 1:1000. An immunoreactive band around ~37 kDa was observed.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intra cellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane- spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR119 is a 335 amino acid protein that is mainly expressed in the pancreas. It is an endogenous receptor for lysophosphatidylcholine (LPC), which is is a lipid mediator involved in insulin secretion from pancreatic ? cells. GPR119 may participate in this insulin secretion, suggesting that it may be potential target for new anti-diabetic drugs.
  Positive Control: MS1 cells
  Cellular Location: Membrane
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A synthetic peptide corresponding to the extracellular domain of mouse GPR 119 protein. This sequence is identical to rat.
• Purification: Affinity Purified
• Concentration: 100ug/200ul
• Specificity: GPR119
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Chu et al. A Role for beta-Cell-Expressed GPR119 in Glycemic Control by Enhancing Glucose-Dependent Insulin Release.
  Endocrinology.148 (6), 2601-2609, (2007).
  

Target Info

• Gene Symbol: GPR119
• Gene Full Name: G protein-coupled receptor 119
• Alias Symbols: glucose-dependent insulinotropic receptor;GPCR2;G-protein coupled receptor 2.
• NCBI Gene Id: 139760
• Description of Target: G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intra cellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane- spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR119 is a 335 amino acid protein that is mainly expressed in the pancreas. It is an endogenous receptor for lysophosphatidylcholine (LPC), which is is a lipid mediator involved in insulin secretion from pancreatic ? cells. GPR119 may participate in this insulin secretion, sμggesting that it may be potential target for new anti-diabetic drμgs.
  
• Uniprot ID: Q8TDV5
• Molecular Weight: 37