Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)

Catalog No: OAEB01821 (Formerly GWB-9AE490)

Size:100UG

OAEB01821 (1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-WB, IP
• Additional Information: Important Note: Please note that, in contrast to other anti-Pericentrin antibodies available, this Ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies).
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: NUP85
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat
• Peptide Sequence: C-ARAIIREGSLEGS
• Concentration: 0.5 mg/ml
• Application Info: Peptide ELISA: antibody detection limit dilution 1:32,000
  WB: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120). Recommended concentration: 1-3 ug/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Toda E et al, J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162.
  Immunoprecipitation: This antibody was successfully used in IP on Human: Toda E et al, J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162.
• Reference: This antibody has been successfully used in WB and IP on Human:
  Toda E, Terashima Y, Sato T, Hirose K, Kanegasaki S, Matsushima K.
  FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration.J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162

Target Info

• Gene Symbol: NUP85|Pcnt
• Gene Full Name: nucleoporin 85|pericentrin (kendrin)
• Alias Symbols: AW476095;C86676;FROUNT;KEN;kendrin;m239;m239Asp;m275;m275Asp;NPHS17;nuclear pore complex protein Nup85;nucleoporin 85kDa;nucleoporin Nup75;nucleoporin Nup85;Nup75;Pcn;Pcnt2;pericentrin;pericentrin 2;pericentrin-250;pericentrin-360.
• NCBI Gene Id: 18541|79902
• Protein Name: Nuclear pore complex protein Nup85
• Uniprot ID: Q9BW27
• Protein Accession #: NP_079120.1
• Molecular Weight: 75