Goat Anti-Mouse IgM Antibody (OASB01475)

Catalog No: OASB01475

Size:1mg

ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM-UNLB followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP.

Product Info

• Tested Species Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Application: FC, ELISPOT, IHC-F, IHC-P, ICC, WB, STIM
• Additional Information: Description: Pooled antisera from goats hyperimmunized with mouse IgM; purified by affinity chromatography on mouse IgM covalently linked to agarose; reacts with the heavy chain of mouse IgM; cross-adsorbed against mouse IgG and IgA for minimal reactivity
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Mouse IgM
• Purification: Affinity chromatography on mouse IgM covalently linked to agarose.
• Concentration: 1.0 mg/mL
• Specificity: IgM
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flowcytometry for conformance to characteristics of a standard reference reagent.
• Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Immunofluorescence:
  ELISA:
  FITC, TRITC, TXRD and BIOT conjugates
  R-PE conjugate
  AP conjugate
  HRP conjugate
  BGAL conjugate
  BIOT conjugate
  <= 1 ug/10⁶ cells
  <= 0.1 ug/10⁶ cells
  1:2,000-1:4,000
  1:4,000-1:8,000
  1:500
  1:5,000-1:20,000
• Application Info: Direct immunofluorescent staining of IgM+ mouse B lymphocytes, Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunoblotting
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 1.0 mgin 1.0 mL PBS/NaN3. Store at 2- 8 C
  - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
  - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
  - The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.5 mg in 1.0 mL or 0.25 mg in 0.5 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
• Cross Absorption: Mouse IgG1, IgG2a, IgG2b, IgG3 and IgA.

Target Info

• Gene Symbol: IGHM
• Gene Full Name: Ig mu chain C region
• Alias Symbols: Ig, Igm, muH, Igh-, Igh6, Igh-6, Igh-M, AI326478
• NCBI Gene Id: 16019
• Uniprot ID: P01872