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| Datasheets/Manuals | Printable datasheet for Goat Anti-MID2 / TRIM1 Antibody (OAEB02432) |
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| Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Porcine|Rat |
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| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB, IHC |
| Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | MID2 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Dog: 92%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 84%; Rat: 92%; Zebrafish: 84% |
| Peptide Sequence | QESPYVSGMKTCH |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 80kDa band observed in mouse heart lysates (calculated MW of 81.3kDa according to NP_036348.1 and 77.9kDa according to NP_438112.1). Recommended concentration: 1-3ug/ml. Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10ug/ml. |
| Reference | * Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B.; MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development.; Hum Mol Genet. 1999 Aug;8(8):1397-407.; Pubmed 10400986 |
| Gene Symbol | Mid2|MID2 |
|---|---|
| Gene Full Name | midline 2 |
| Alias Symbols | FXY2;midin 2;midline defect 2;MRX101;probable E3 ubiquitin-protein ligase MID2;RING finger protein 60;RING-type E3 ubiquitin transferase MID2;RNF60;Tri;TRIM1;tripartite motif protein 1;tripartite motif-containing protein 1;XLID101. |
| NCBI Gene Id | 11043|23947 |
| Protein Name | Probable E3 ubiquitin-protein ligase MID2 |
| Uniprot ID | Q9UJV3 |
| Protein Accession # | NP_036348.2 |
| Molecular Weight | 83.2, 79.9 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-MID2 / TRIM1 Antibody (OAEB02432)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Porcine|Rat".
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How long will it take to receive "Goat Anti-MID2 / TRIM1 Antibody (OAEB02432)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-MID2 / TRIM1 Antibody (OAEB02432)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-MID2 / TRIM1 Antibody (OAEB02432)"?
This target may also be called "FXY2;midin 2;midline defect 2;MRX101;probable E3 ubiquitin-protein ligase MID2;RING finger protein 60;RING-type E3 ubiquitin transferase MID2;RNF60;Tri;TRIM1;tripartite motif protein 1;tripartite motif-containing protein 1;XLID101." in publications.
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What is the shipping cost for "Goat Anti-MID2 / TRIM1 Antibody (OAEB02432)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-MID2 / TRIM1 Antibody (OAEB02432)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-MID2 / TRIM1 Antibody (OAEB02432)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "83.2, 79.9".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-MID2 / TRIM1 Antibody (OAEB02432)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Mid2|MID2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Mid2|MID2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Mid2|MID2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Mid2|MID2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Mid2|MID2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Mid2|MID2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
