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Datasheets/Manuals | Printable datasheet for Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395) |
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Predicted Species Reactivity | Bovine|Dog|Human|Mouse|Porcine|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB, IHC |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | EIF4ENIF1 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 91% |
Peptide Sequence | C-AKVISVDELEYRQ |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below)). Recommended for use at 0.25-0.5ug/ml Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10ug/ml. |
Reference | * Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N.; A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E.; EMBO J. 2000 Jun 15;19(12):3142-56.; Pubmed 10856257 |
Gene Symbol | Eif4enif1|EIF4ENIF1 |
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Gene Full Name | eukaryotic translation initiation factor 4E nuclear import factor 1 |
Alias Symbols | 2610509L04Rik;4E-T;4E-transporter;A930019J01Rik;AA410001;AU021239;CD40 ligand-activated specific transcript 4;Cla;Clast4;D11Ertd166;D11Ertd166e;eIF4E transporter;eukaryotic translation initiation factor 4E transporter. |
NCBI Gene Id | 56478|74203 |
Protein Name | Eukaryotic translation initiation factor 4E transporter |
Uniprot ID | Q9NRA8 |
Protein Accession # | NP_062817.2 |
Molecular Weight | 108; 88.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Human|Mouse|Porcine|Rat".
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How long will it take to receive "Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395)"?
This target may also be called "2610509L04Rik;4E-T;4E-transporter;A930019J01Rik;AA410001;AU021239;CD40 ligand-activated specific transcript 4;Cla;Clast4;D11Ertd166;D11Ertd166e;eIF4E transporter;eukaryotic translation initiation factor 4E transporter." in publications.
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What is the shipping cost for "Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "108; 88.2".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-4E-T / EIF4ENIF1 Antibody (OAEB02395)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Eif4enif1|EIF4ENIF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Eif4enif1|EIF4ENIF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Eif4enif1|EIF4ENIF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Eif4enif1|EIF4ENIF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Eif4enif1|EIF4ENIF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Eif4enif1|EIF4ENIF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.