GLS2 ELISA Kit (Human) (OKEH07960)

Catalog No: OKEH07960

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology GLS2 ELISA Kit (Human) (OKEH07960) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLS2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GLS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GLS2 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.5% (n = 20)
  Mean Inter-assay CV%: <=11.9% (n = 20)
• ELISA Kit Component:
  • GLS2 Microplate: 96 Wells (12 x 8 Well strips)
  • GLS2 Lyophilized Standard: 2
  • 100X Biotinylated GLS2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
  Subcellular Location: Mitochondrion
  Unigene: Hs.212606
  SMR: Q9UI32
  String: 9606.ENSP00000310447
  KEGG: hsa:27165
  MIM: 606365
  Pfam: PF12796
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39.4pg/mL
• Specificity: Natural and recombinant Human Glutaminase liver isoform, mitochondrial
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GLS2
• Gene Full Name: glutaminase 2
• Alias Symbols: breast cell glutaminase;GA;GLS;glutaminase 2 (liver, mitochondrial);glutaminase I;glutaminase liver isoform, mitochondrial;hLGA;LGA;L-glutaminase;L-glutamine amidohydrolase;phosphate-activated glutaminase;phosphate-dependent glutaminase.
• NCBI Gene Id: 27165
• Protein Name: Q9UI32
• Description of Target: Glutaminase liver isoform, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UI32
• Protein Accession #: NP_001267727.1
• Nucleotide Accession #: NM_001280798.1