GLA Chemi-Luminescent ELISA Kit (Human) (OKCD03534)

Catalog No: OKCD03534

Size:96WELLS

Human GLA ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-90%	79-91%	97-105%	80-94%
  EDTA Plasma (n=5)	80-105%	91-99%	90-98%	82-93%
  Heparin Plasma (n=5)	97-105%	78-103%	86-103%	85-99%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Galactosidase Alpha (GLa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Galactosidase Alpha (GLa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Galactosidase Alpha (GLa) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	91-105	96
  EDTA Plasma (n=5)	88-98	92
  Heparin Plasma (n=5)	95-102	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galactosidase Alpha (GLa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galactosidase Alpha (GLa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • GLA Microplate: 96 Wells (12 x 8 Well strips)
  • GLA Lyophilized Standard: 2
  • 100X GLA Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GLA
• Gene Full Name: galactosidase alpha
• Alias Symbols: agalsidase alfa;alpha-D-galactosidase A;Alpha-D-galactoside galactohydrolase;alpha-D-galactoside galactohydrolase 1;alpha-gal A;alpha-galactosidase A;GALA;galactosylgalactosylglucosylceramidase GLA;melibiase.
• NCBI Gene Id: 2717
• Protein Name: Alpha-galactosidase A
• Description of Target: Alpha-galactosidase A
• Uniprot ID: P06280
• Protein Accession #: NP_000160
• Nucleotide Accession #: NM_000169