GIT2 Antibody (OAGA05414)

Catalog No: OAGA05414

Size:100 ul

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] diluted at 1:1000.

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GIT2 antibody [N1N3] diluted at 1:1000.

GIT2 antibody [N1N3] detects GIT2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: GIT2 protein stained by GIT2 antibody [N1N3] diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI .

GIT2 antibody [N1N3] detects GIT2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GIT2 protein stained by GIT2 antibody [N1N3] diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-F, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GIT2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.81 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-Fr: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: GIT2
• Gene Full Name: GIT ArfGAP 2
• Alias Symbols: PKL, CAT2, CAT-2
• NCBI Gene Id: 9815
• Protein Name: ARF GTPase-activating protein GIT2
• Description of Target: This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.
• Uniprot ID: Q14161
• Protein Accession #: NP_001128685.1
• Nucleotide Accession #: NM_001135213.2