Catalog No: OKEH03744
Size:96T
Price: $625.00
SKU
OKEH03744
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology GIN1 ELISA Kit (Rat) (OKEH03744) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Gin1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Gin1 and incubated. The Gin1 found in the sample or standards competes with the biotinylated Gin1 for limited binding sites on the immobilized anti-human Gin1 antibody. Excess unbound biotinylated Gin1 and sample or standard Gin1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Gin1 captured in the well and inversely proportional to the amount of Gin1 which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/ml
ELISA Kit Component
ComponentAmount
Anti-Gin1 Microplate96 Wells (12 x 8 Well strips)
Gin1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Gin1-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.397 ng/mL
SpecificityNatural and recombinant Rat Gypsy retrotransposon integrase-like protein 1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolGin1
Gene Full Namegypsy retrotransposon integrase 1
Alias SymbolsGIN-1;gypsy retrotransposon integrase-like protein 1;RGD1307449.
NCBI Gene Id316687
Protein NameQ66H30
Description of TargetGypsy retrotransposon integrase-like protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q66H30
Protein Accession #NP_001020183.1
Nucleotide Accession #NM_001025012.1
Protein Size (# AA)518
Molecular Weight59 kDa
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