GHRH ELISA Kit (Mouse) (OKEH03121)

Catalog No: OKEH03121

Size:96T

Mouse GHRH ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology GHRH ELISA Kit (Mouse) (OKEH03121) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ghrh has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ghrh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ghrh captured in well.
• ELISA Kit Range: 0.39-25ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.2% (n = 20)
  Mean Inter-assay CV%: < 8.8% (n = 20)
• ELISA Kit Component:
  • Anti-Ghrh Microplate: 96 Wells (12 x 8 Well strips)
  • Ghrh Lyophilized Standard: 2
  • 100X Biotinylated Ghrh Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.195 ng/mL
• Specificity: Natural and recombinant Mouse Somatoliberin
• Publications:

  Repeated hypoglycemia remodels neural inputs and disrupts mitochondrial function to blunt glucose-inhibited GHRH neuron responsiveness. JCI Insight. 5, NULL (2020). 33148883

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for GHRH ELISA Kit (Mouse) (OKEH03121) on CiteAb

Target Info

• Gene Symbol: Ghrh
• Gene Full Name: growth hormone releasing hormone
• Alias Symbols: Ghr;Ghrf;GRF;growth hormone-releasing factor;Growth hormone-releasing hormone;somatoliberin.
• NCBI Gene Id: 14601
• Protein Name: P16043
• Description of Target: Somatoliberin
• Uniprot ID: https://www.uniprot.org/uniprot/P16043
• Protein Accession #: NP_034415.1
• Nucleotide Accession #: NM_010285.2
• Protein Size (# AA): 103
• Molecular Weight: 12 kDa