Fyn Antibody (Phospho-Tyr530) (OAAF00077)

Catalog No: OAAF00077 (Formerly GWB-ASB106)

Size:100 ug

Western blot analysis of extracts from 293 cells treated with H2O2 100uM 15', using Fyn (Phospho-Tyr530) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Fyn (Phospho-Tyr530) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Fyn around the phosphorylation site of Tyr530.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Fyn (Phospho-Tyr530) antibody detects endogenous levels of Fyn only when phosphorylated at tyrosine 530.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:1000
• Reference: Weijie Li, J. Biol. Chem., Jan 2007; 282: 1161 - 1169.
  Xiaowu Li, J. Biol. Chem., Oct 2003; 278: 41646 - 41653.
  Mamiko Maekawa, Biol Reprod, Jan 2002; 66: 211.
  Jeannie Chin, J. Neurosci., Oct 2005; 25: 9694 - 9703.

Target Info

• Gene Symbol: FYN
• Gene Full Name: FYN proto-oncogene, Src family tyrosine kinase
• Alias Symbols: c-syn protooncogene;FYN oncogene related to SRC, FGR, YES;OKT3-induced calcium influx regulator;p59-FYN;proto-oncogene c-Fyn;proto-oncogene Syn;SLK;src/yes-related novel;src-like kinase;SYN;tyrosine kinase p59fyn(T);tyrosine-protein kinase Fyn.
• NCBI Gene Id: 2534
• Protein Name: Tyrosine-protein kinase Fyn
• Description of Target: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.
• Uniprot ID: P06241
• Protein Accession #: NP_002028.1
• Molecular Weight: 60 kDa