Furazolidone ELISA Kit (OKAO00101)

Catalog No: OKAO00101

Size:96 Wells

General Furazolidone ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: General
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology Furazolidone ELISA Kit (OKAO00101) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Furazolidone has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Furazolidone antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
• ELISA Kit Range: 0.02-1.62 ppb
• ELISA Kit Recovery:

  Sample	Amount
  Tissue, Liver	90% +/- 15%
  Honey, milk	80% +/- 15%
  Intestine	80% +/- 15%

• ELISA Kit Reproducibility: Mean Intra-Assay: CV<8% (n= 3 x 20)
  Mean Inter-Assay: CV<12% (n= 3 x 40)
• ELISA Kit Component:
  • Furazolidone Microplate: 96 Wells (12 x 8 Well Strips)
  • Furazolidone Standards: 6 x 1 mL
  • Furazolidone Rabbit Detector Antibody: 1 x 7 mL
  • Anti-Rabbit / HRP Conjugate: 1 x 7 mL
  • Detection Reagent A: 1 x 7 mL
  • Detection Reagent B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
  • 20X Wash Buffer: 2 x 40 mL
  • 2X Sample Prep Buffer: 2 x 20 mL
  • 2-Nitrobenzaldehyde (C7H5NO3): 1 x 10 mL
• Additional Information: CAS: 67-45-8
  PubChem: 5323714
  Molecular Formula: C8H7N3O5
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue (Chicken, duck, porcine meat, beef and mutton, fish, shrimp), liver (Chicken, duck, porcine meat, beef and mutton), honey, milk, intestine.
• Sensitivity:

  Sample	Amount
  Tissue	0.1 ppb
  Liver	0.1 ppb
  Honey, milk	0.1 ppb
  Intestine	0.1 ppb

• Specificity:

  Substance	Cross Reactivity Rate
  AOZ	100%
  AHD	<0.1%
  AMOZ	<0.1%
  SEM	<0.1%

• Assay Info: Assay Methodology: Competitive Inhibition ELISA

Target Info

• Alias Symbols: AOZ, Furazolidone, Furazolidine, 67-45-8, Furoxone, Nitrofurazolidone, Nifurazolidone
• Protein Name: Furazolidone
• Description of Target: A nitrofuran derivative with antiprotozoal and antibacterial activity. Furazolidone acts by gradual inhibition of monoamine oxidase. (From Martindale, The Extra Pharmacopoeia, 30th ed, p514)
• Molecular Weight: 225.15828 g/mol