FSHB ELISA Kit (rabbit) (OKCA00938)

Catalog No: OKCA00938

Size:96 Wells

rabbit FSHB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: rabbit
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	85	80-92
  1:2	99	91-105
  1:4	103	92-110
  1:8	94	86-98

• ELISA Kit Principle: Aviva Systems Biology FSHB ELISA Kit (rabbit) (OKCA00938) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for FSHB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FSHB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FSHB captured in well.
• ELISA Kit Range: 1.56 mIU/mL-100 mIU/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94	89-98
  EDTA Plasma (n=4)	96	90-105

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level FSHB were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level FSHB were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • FSHB Microplate: 96 Wells (12 x 8 Well strips)
  • FSHB Lyophilized Standard: 2
  • 100X Biotinylated FSHB Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.39 mIU/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FSHB
• Gene Full Name: Follitropin subunit beta
• Alias Symbols: FSH
• NCBI Gene Id: 100008946
• Protein Name: Follitropin subunit beta
• Description of Target: Stimulates development of follicle and spermatogenesis in the reproductive organs.
• Uniprot ID: Q6IY73
• Protein Accession #: NP_001075640.1
• Nucleotide Accession #: NM_001082171.2