FGFR4 ELISA Kit (Human) (OKDD00266)

Catalog No: OKDD00266 (Formerly GWB-KBBQY8)

Size:96 Wells

Human FGFR4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology FGFR4 ELISA Kit (Human) (OKDD00266) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for FGFR4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGFR4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample FGFR4 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGFR4 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGFR4 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-FGFR4 Microplate: 96 Wells (12 x 8 Well Strips)
  • FGFR4 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X FGFR4 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.041 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGFR4. No significant cross-reactivity or interference between FGFR4 and analogues was observed.
• Publications:

  Tumor Fibroblast Growth Factor Receptor 4 Level Predicts the Efficacy of Lenvatinib in Patients With Advanced Hepatocellular Carcinoma. Clin Transl Gastroenterol. 11, e00179 (2020). 32677805

  Validation of a Novel Modified Aptamer-Based Array Proteomic Platform in Patients with End-Stage Renal Disease. Diagnostics (Basel). 8, (2018). 30297602

• Assay Info: Quantitative Sandwich ELISA
• Description:
  View product citations for FGFR4 ELISA Kit (Human) (OKDD00266) on CiteAb

Target Info

• Gene Symbol: FGFR4
• Gene Full Name: fibroblast growth factor receptor 4
• Alias Symbols: CD334;fibroblast growth factor receptor 4;hydroxyaryl-protein kinase;JTK2;protein-tyrosine kinase;TKF;tyrosine kinase related to fibroblast growth factor receptor;tyrosylprotein kinase.
• NCBI Gene Id: 2264
• Protein Name: Fibroblast growth factor receptor 4
• Description of Target: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
• Uniprot ID: P22455
• Protein Accession #: NP_001278909.1
• Nucleotide Accession #: NM_001291980.1
• Protein Size (# AA): 802
• Molecular Weight: 88 kDa