FGFR2 Antibody - N-terminal region (OAAB17154)

Catalog No: OAAB17154 (Formerly GWB-U8DW16)

Size:400ul

FGFR2 Antibody (N-term) (Cat. # OAAB17154) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).

FGFR2 is strongly supported by BioGPS gene expression data to be expressed in Hela

FGFR2 Antibody (N-term) (Cat. # OAAB17154) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).

Western blot analysis of lysates from Hela, K562, MCF-7 cell line (from left to right), using FGFR2 Antibody (Cat. #OAAB17154). OAAB17154 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term)(Cat#OAAB17154). OAAB17154 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term)(Cat#OAAB17154). OAAB17154 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

FGFR2 Antibody (N-term) (Cat. # OAAB17154) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of FGFR2 Antibody (N-term) (Cat# OAAB17154) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, IF, FC
• Additional Information: Cellular Location: Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cytoplasmic vesicle. Note=Detected on osteoblast plasma membrane lipid rafts. After ligand binding, the activated receptor is rapidly internalized and degraded Isoform 3: Cell membrane; Single-pass type I membrane protein. Note=After ligand binding, the activated receptor is rapidly internalized and degraded Isoform 19: Secreted.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human FGFR2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation: FGFR2 is strongly supported by BioGPS gene expression data to be expressed in Hela
• Intended Use: FGFR2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: IF: 1:25
  WB: 1:1000~2000
  IHC-P: 1:50~100
  FC: 1:25

Target Info

• Gene Symbol: FGFR2
• Alias Symbols: BEK, JWS, BBDS, CEK3, CFD1, ECT1, KGFR, TK14, TK25, BFR-1, CD332, K-SAM
• NCBI Gene Id: 2263
• Protein Name: Fibroblast growth factor receptor 2
• Description of Target: FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.
• Uniprot ID: P21802
• Protein Accession #: NP_000132.3
• Molecular Weight: 92 kDa