FGF6 ELISA Kit (Mouse) (OKCD04357)

Catalog No: OKCD04357

Size:96WELLS

Mouse FGF6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-105%	93-101%	83-90%	82-97%
  EDTA Plasma (n=5)	82-103%	98-105%	97-105%	98-105%
  Heparin Plasma (n=5)	93-101%	94-103%	92-104%	81-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 6 (FGF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 6 (FGF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 6 (FGF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibroblast Growth Factor 6 (FGF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	83-105	101
  EDTA Plasma (n=5)	95-105	101
  Heparin Plasma (n=5)	80-101	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 6 (FGF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Fgf6 Microplate: 96 Wells (12 x 8 Well strips)
  • Fgf6 Lyophilized Standard: 2
  • 100X Fgf6 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: 6.1 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fgf6
• Gene Full Name: fibroblast growth factor 6
• Alias Symbols: Fgf-6;Fgf7b;fibroblast growth factor 6;fibroblast growth factor 7b;HBGF-6;heparin secretory-transforming protein 2;heparin-binding growth factor 6;HST-2;HSTF-2.
• NCBI Gene Id: 14177
• Protein Name: Fibroblast growth factor 6
• Description of Target: Plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis, and is required for normal muscle regeneration.
• Uniprot ID: P21658
• Protein Accession #: NP_034334.1
• Nucleotide Accession #: NM_010204.1