FASL Chemi-Luminescent ELISA Kit (Mouse) (OKCD03448)

Catalog No: OKCD03448

Size:96WELLS

Mouse FASL ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-91%	81-91%	84-101%	98-105%
  EDTA Plasma (n=5)	91-98%	94-102%	96-105%	78-104%
  Heparin Plasma (n=5)	83-95%	80-91%	84-103%	83-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis Ligand (FASL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Factor Related Apoptosis Ligand (FASL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Factor Related Apoptosis Ligand (FASL) level in the sample or standard.
• ELISA Kit Range: 41.2-30,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-102	97
  EDTA Plasma (n=5)	80-99	84
  Heparin Plasma (n=5)	84-99	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis Ligand (FASL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Fasl Microplate: 96 Wells (12 x 8 Well strips)
  • Fasl Lyophilized Standard: 2
  • 100X Fasl Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 41.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fasl
• Gene Full Name: Fas ligand (TNF superfamily, member 6)
• Alias Symbols: APT1;APT1LG1;CD178;CD95;CD95 ligand;CD95L;CD95-L;F;Fa;Fas antigen ligand;Fas-L;Faslg;generalized lymphoproliferative disease;gld;Tnfs;Tnfsf6;Tnlg1a;tumor necrosis factor ligand 1a;tumor necrosis factor ligand superfamily member 6.
• NCBI Gene Id: 14103
• Protein Name: Fas ligand (TNF superfamily, member 6)
• Description of Target: Fas ligand (TNF superfamily, member 6)
• Uniprot ID: Q544E9
• Protein Accession #: NP_034307
• Nucleotide Accession #: NM_010177