FANCA ELISA Kit (Human) (OKEH08037)

Catalog No: OKEH08037

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FANCA ELISA Kit (Human) (OKEH08037) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FANCA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FANCA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FANCA captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.5% (n = 20)
  Mean Inter-assay CV%: <=6.9% (n = 20)
• ELISA Kit Component:
  • FANCA Microplate: 96 Wells (12 x 8 Well strips)
  • FANCA Lyophilized Standard: 2
  • 100X Biotinylated FANCA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.
  Subcellular Location: Nucleus Cytoplasm The major form is nuclear. The minor form is cytoplasmic.
  Unigene: Hs.744083
  String: 9606.ENSP00000373952
  KEGG: hsa:2175
  MIM: 227650
  Pfam: PF03511
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 7.65pg/mL
• Specificity: Natural and recombinant Human Fanconi anemia group A protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FANCA
• Gene Full Name: FA complementation group A
• Alias Symbols: FA;FA1;FAA;FACA;FAH;FA-H;FANCH;Fanconi anemia complementation group A;Fanconi anemia group A protein;Fanconi anemia, complementation group H;Fanconi anemia, type 1.
• NCBI Gene Id: 2175
• Protein Name: O15360
• Description of Target: Fanconi anemia group A protein
• Uniprot ID: https://www.uniprot.org/uniprot/O15360
• Protein Accession #: NP_000126.2
• Nucleotide Accession #: NM_000135.2